β-Nicotinamide Adenine Dinucleotide Reduced, Disodium Salt

β-Nicotinamide Adenine Dinucleotide Reduced, Disodium Salt

Synonyms β-DPNH; β-NADH, 2Na; DPNH; Diphosphopyridine nucleotide reduced form; NADH ; Reduced DPN; Cozymase, reduced form; Dihydrophosphopyridine nucleotide, reduced form; Dihydronicotinamide adenine dinucleotide

CAS Number: 606-68-8 Molecular Formula: C21H27N7O14P2Na2 Molecular Weight: 709.409 g/mol

EC Number: 210-123-3 MDL Number: MFCD03791273

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SKU 02101168-CF
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Description

Product Description

β-Nicotinamide Adenine Dinucleotide Reduced, Disodium Salt

Application Notes

β-Nicotinamide adenine dinucleotide (NAD+) and β-Nicotinamide adenine dinucleotide, reduced (NADH) comprise a coenzyme redox pair (NAD+:NADH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. In addition to its redox function, NAD+/NADH is a donor of ADP-ribose units in ADP-ribosylaton (ADP-ribosyltransferases; poly(ADP-ribose) polymerases ) reactions and a precursor of cyclic ADP-ribose (ADP-ribosyl cyclases). Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NADH as a cofactor and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. β-NADH is fluorescent; whereas, β-NAD+ is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10−7 M. Discussion of optimizing the fluorescence intensity and identification of interfering substances has been reported.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Coenzyme

Specifications
SKU 02101168-CF
Application Notes β-Nicotinamide adenine dinucleotide (NAD+) and β-Nicotinamide adenine dinucleotide, reduced (NADH) comprise a coenzyme redox pair (NAD+:NADH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. In addition to its redox function, NAD+/NADH is a donor of ADP-ribose units in ADP-ribosylaton (ADP-ribosyltransferases; poly(ADP-ribose) polymerases ) reactions and a precursor of cyclic ADP-ribose (ADP-ribosyl cyclases). Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NADH as a cofactor and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. β-NADH is fluorescent; whereas, β-NAD+ is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10−7 M. Discussion of optimizing the fluorescence intensity and identification of interfering substances has been reported.
Biochemical Physiological Actions NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, beta-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease. As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.
EC Number 210-123-3
Extinction Coefficient EmM = 6.22 (340 nm) and 14.4 (259 nm, pH 9.5)(Lit.)
Format powder
Applications Coenzyme
Molecular Formula C21H27N7O14P2Na2
Molecular Weight 709.409 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance White Powder
Product Families Description β-Nicotinamide-adenine dinucleotide reduced
Product Overview β-Nicotinamide Adenine Dinucleotide Reduced, Disodium Salt
Purity ≥98%
Storage and Handling Store at -0 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance Water: λ max: 258 - 260 nm