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LymphoSep™ - Lymphocyte Separation Medium

LymphoSep™ - Lymphocyte Separation Medium

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SKU 0916922-CF
Price:
A$39.50
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Description

Product Description

Lymphosep - Lymphocyte Separation Medium is a sterile, iso-osmotic, low viscosity solution for density centrifugation yielding a clear separation of lymphocytes from heparinized or defibrinated human blood. Lymphocytes are isolated by layering diluted, heparinized blood over a solution of sodium metrizoate that contains a polysaccharide. This polysaccharide aggregates the erythrocytes so that they can be sedimented by low centrifugation. The less dense lymphocytes remain as a clearly defined band at the plasma Lymphosep interface.

  • Maximum yield of mononuclear cells
  • Easy one-step centrifugation
  • >96% cell viability of lymphocytes
  • Low endotoxins
  • Autoclave sterilization

Check out our latest article in Genetic Engineering & Biotechnology News about High-Performance Density Gradient Media for Purification of Leukocytes from Blood.

Application Notes

LSM is formulated for isolation of mononuclear cells from defibrinated or heparinized whole human blood. One-step centrifugation permits separation of mononuclear lymphocytes from erythrocytes, polynuclear lymphocytes and most platelets.

Key Applications

In vitro isolation of lymphocytes from diluted whole blood.

Specifications
SKU 0916922-CF
Application Notes LSM is formulated for isolation of mononuclear cells from defibrinated or heparinized whole human blood. One-step centrifugation permits separation of mononuclear lymphocytes from erythrocytes, polynuclear lymphocytes and most platelets.
Formulation Details Liquid - Lymphosep is supplied as a ready made sterile aqueous solution of sodium metrizoate (9.6% w/v) and Ficoll® (5.6% w/v).
Applications In vitro isolation of lymphocytes from diluted whole blood.
Pack Size No
pH 6.0 - 9.0
Physical Appearance Clear colorless liquid
Presentation Lymphosep® is supplied as a ready made sterile aqueous solution of sodium metrizoate (9.6% w/v) and Ficoll® (5.6% w/v). | Liquid
Product Families Description No
Product Overview Ready to use quick, simple and reliable medium for the isolation of lymphocytes from whole blood. Lymphocytes are isolated by layering diluted, heparinized blood over a solution of sodium metrizoate that contains a polysaccharide. This polysaccharide aggregates the erythrocytes so that they can be sedimented by low centrifugation. The less dense lymphocytes remain as a clearly defined band at the plasma Lymphosep® interface.
Sterility Sterile
Storage and Handling Store at Room temperature (15-30°C); protect from light