CIRCLEGROW (STERILE SOLUTION)
Circlegrow media is used for growth of E.coli and extends plasmid production past log phase.
Circlegrow™ is designed for rapid growth of E. coli and high yields of plasmid DNA.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation
|Application Notes||Circlegrow™ is designed for rapid growth of E. coli and high yields of plasmid DNA.|
|Cells Tested||E. coli|
|Certifications||Molecular Biology Certified™|
|Clarity NTU||Clear once disolved in water|
|Media Type||Bacterial Media|
|Formulation Details||Proprietary formulation containing Yeast Extract; Tryptone Broth; and Vitamins. Biological buffers have been added for proper pH adjustment.|
|Grade||Molecular Biology Certified™ Bacterial Growth Medium|
|Applications||Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation|
|Kit Compatibility||Any Plasmid Purification Kit including MP's RapidPURE™, RPM™, and PhoenIX™ Lines|
|Pack Size||500 mL|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|pH||Acceptable pH range is 6.9 ± 0.3 at 25 °C. Please note that the pH of CIRCLEGROW™ Medium liquid increases as the temperature of the solution decreases.|
|Preparation Method||Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in Circlegrow formulations. Chosen reagents are combined in the accurate proportions according to the proprietary Circlegrow formula and thoroughly blended for a uniform distribution.|
|Product Overview||At the end of log phase, cells growing in Circlegrow begin to utilize a proprietary second carbon source that becomes available on a delayed basis. Cell growth and plasmid replication continue long after cells in conventional media have reached stationary phase or expired. Bacteria commonly grow in CIRCLEGROW to a density triple that achieved in other media. The slow post-log phase growth of bacteria in Circlegrow eliminates the need for constant OD monitoring. Circlegrow cultures left overnight or over the weekend will have a much lower percentage of dead cells, and a higher percentage of plasmid DNA, than cultures prepared with any other media. The result is a higher yield of plasmid DNA in less volume with less attention.|
|Recommended Replacement For||LB Media|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology|
|Storage and Handling||Store dry CIRCLEGROW at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.|