Culture Media, Powder
Terrific Broth, Modified
Terrific Broth, Modified is a buffered enriched medium for the propagation of recombinant E. coli strains. The medium was developed by Tartoff and Hobbs. Recombinant strains have an extended growth phase in the medium. The addition of extra Tryptone and Yeast Extract in the medium allows higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid. Terrific Broth, Modified has a lower concentraion of Dibasic Potassium Phosphate than Terrific Broth.
Tryptic Soy Agar
Tryptic Soy Agar is a medium very rich in nutrients for "general use" in microbiological laboratories. Containing two peptones obtained by enzymatic hydrolysis of casein and soy protein, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. If desired, antibiotics can easily be incorporated as well as other supplements or inhibitory agents. A short list of microorganisms that grow on this medium are the following: Streptococcus, Neisseria, Brucella, Corynebacterium, Listeria, Pasteurella, Vibrio, Haemophilus vaginals, Candida, etc.
Tryptic Soy Broth
Tryptic Soy Broth is used for the cultivation of fastidious and non-fastidious microorganisms. The inclusion of casein peptone and soy peptone to the formula enhances the growth of many fastidious microorganisms without the addition of blood or serum. It can support the growth of a wide variety of microorganisms including aerobes, anaerobes and fungi. Because of its nutritional capabilities it can be used as an enrichment broth and as a blood culture medium.
Tryptone is a pancreatic digest of casein. Tryptone is utillized as a source of nitrogen in many culture media for growing bacteria as well as fungi. The lack of detectable carbohydrates makes this peptone an excellent choice for bacterial studies based on fermentation reactions. Its high level of tryptophan makes it useful in the production of indol. This preparation supplements media for the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as E. coli and Pseudomonas species. It is used to supplement media in the cultivation of anaerobes (Clostridia and other fermenting organisms, e.g., Lactobacilli).
Williams Medium E, Powder, With L-Glutamine, Without Sodium Bicarbonate
Isolated epithelial cells, as described in his sequential plating method by Williams, et al., in 1971, were originally cultivated in a rich medium known as Williams' Medium D. From these early newborn animal studies, Williams and Gunn developed a subsequent medium, Williams' Medium E, for the long-term cultivation of adult rat liver epithelial cells.