Dino

Cloning & Transformation

6 Results Found
11XGAL0100.png

}{X-GAL, MOLECULAR BIOL. GRADE

X-Gal (5-Bromo-4-Chloro-3-Indolyl-AY-D-galactopyranoside) is a β-galactosidase substrate. In the presence of β-galactosidase, X-Gal is hydrolyzed and yields a blue precipitate.

EZ-YEAST TRANSFORMATION KIT

- Simple and fast with minimal hands-on time - No need to make cells competent prior to transformation - High throughput: can be easily adapted to 96-well format - Ideal for simultaneous transformation of library and bait vectors into yeast two-hybrid reporter strains - Transformation efficiency up to 1 x 103 colonies per μg DNA

11IPTG0001.png

}{IPTG

IPTG (Isopropyl-ß-D-thiogalactopyranoside) is a galactose analogue not recognized by ß-galactosidase. IPTG is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli.

Ampicillin Sodium Salt

Ampicillin Sodium Salt

ALKALI-CATION YEAST TRANSFORMA

The Alkali-Cation™ Yeast Transformation Kit provides the reagents needed to perform an easy and quick transformation of yeast, while obtaining a large number of transformants. Its ease of use, robustness, and scalability make this the method of choice for the high-efficiency transformation needed in two hybrid screens. Host cells are made competent by treatment with a lithium/cesium acetate solution. Plasmid DNA is added to the competent cells along with carefully prepared carrier DNA that has been optimized to provide high transformation effi ciencies. The Alkali-Cation™ Yeast Transformation Kit has also been successfully used to transform linear DNA into P. pastoris for protein expression studies.

TRANSFORM & GROW BACTERIAL TRA

- No Heat Shock Required - Transform-It™ Solution is Ready-to-Use - Transform Plasmids, Ligations, and Cosmids - Store Competent Cells 10 Weeks or Longer - More Reliable than “Do-it-Yourself” Methods - Easy Procedure with Minimal Hands-On Time

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